eukaryotic expression plasmid encoding the firefly luciferase gene pgl4.51 [luc2/cmv/neo] (Promega)
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Promega
eukaryotic expression plasmid encoding the firefly luciferase gene pgl4.51 [luc2/cmv/neo]
Eukaryotic Expression Plasmid Encoding The Firefly Luciferase Gene Pgl4.51 [Luc2/Cmv/Neo], supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pgl4%2E51/firefly+luciferase+vector++pgl4+13++luc2+sv40/pmc11844280-39-15-26
Average 90 stars, based on 1 article reviews
Eukaryotic Expression Plasmid Encoding The Firefly Luciferase Gene Pgl4.51 [Luc2/Cmv/Neo], supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pgl4%2E51/firefly+luciferase+vector++pgl4+13++luc2+sv40/pmc11844280-39-15-26
Average 90 stars, based on 1 article reviews
eukaryotic expression plasmid encoding the firefly luciferase gene pgl4.51 [luc2/cmv/neo] - by Bioz Stars,
2026-11
90/100 stars
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Transfection:Article Title: Length-Controlled Nanofiber Micelleplexes as Efficient Nucleic Acid Delivery Vehicles. Article Snippet: Micelleplexes show great promise as effective polymeric delivery systems for nucleic acids.. Although studies have shown that spherical micelleplexes can exhibit superior cellular transfection to polyplexes, to date there has been no report on the effects of micelleplex morphology on cellular transfection.. In this work, we prepared precision, length-tunable poly(fluorenetrimethylenecarbonate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PFTMC16-b-PDMAEMA131) nanofiber micelleplexes and compared their properties and transfection activity to those of the equivalent nanosphere micelleplexes and polyplexes. Article Title: N 6 -methyladenosine in 5' UTR does not promote translation initiation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins GMP-PNP Sigma Cat # G0635 TSY Pacific Biosciences 100-214-900 Cy3 mono hydrazide Cytiva PA13121 Cyanine 3.5 maleimide AAT Bioquest Cat # 149 Cy5 mono hydrazide Cytiva PA15121 2’-O-methyltransferase NEB Cat # M0336 T4 RNA ligase ThermoFisher Scientific Cat # EL0021 T4 RNA ligase 2 NEB Cat # M0242S Terminator 5ʹ-PhosphateDependent Exonuclease Lucigen Cat # TER51020 RNA 50 polyphosphatase Lucigen Cat # 136120 RNasin Ribonuclease Inhibitors Promega Cat # N251B DNase I ThermoFisher Scientific EN0521 MssI FD Therno Scientific Cat # FD1344 Protein kinase R (PKR) Abcam Cat # ab32052 Critical commercial assays T7 megascript kit Invitrogen Cat # AM1334 Vaccinia Capping System NEB Cat # M2080S HiScribe RNA synthesis kit NEB Cat # E2040S OneStep Human Coupled IVT Kit ThermoFisher Scientific Cat # 88881 Luciferase Assay System Promega Cat # Article Title: Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery Article Snippet: Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree.. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction.. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Article Title: In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging Article Snippet: Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways Article Snippet: The transfection with the plasmid pGL4.51 ( Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways. Article Snippet: Objective: The recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) to have a bi-specific oncolytic character in many tumor cells, but its action pathway in killing tumor cells has not been accurately elucidated.. Here, we studied the mechanism of apoptosis and autophagy induced by Ad-VT and the interaction between autophagy Article Title: Suppression effect of a dual cancer-specific oncolytic adenovirus on luciferase-labeled human melanoma cells in vitro and in vivo Article Snippet: BACKGROUND: To explore the suppressive effect of Apoptin-loaded oncolytic adenovirus (Ad-VT) on luciferase-labeled human melanoma cells in vitro and in vivo.. METHODS: The stable luciferase-expressing human melanoma cells A375-luc or M14-luc were obtained by transfecting the plasmid pGL4.51 and selection with G418, followed by luciferase activity, genetic stability and bioluminescence intensity assays.. In vitro, the inhibitory effects of Ad-VT on A375-luc or M14-luc were evaluated using the MTS cell proliferation, FITC-Annexin V apoptosis detection, transwell migration, Matrigel invasion and scratch assays. Plasmid Preparation:Article Title: Length-Controlled Nanofiber Micelleplexes as Efficient Nucleic Acid Delivery Vehicles. Article Snippet: Micelleplexes show great promise as effective polymeric delivery systems for nucleic acids.. Although studies have shown that spherical micelleplexes can exhibit superior cellular transfection to polyplexes, to date there has been no report on the effects of micelleplex morphology on cellular transfection.. In this work, we prepared precision, length-tunable poly(fluorenetrimethylenecarbonate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PFTMC16-b-PDMAEMA131) nanofiber micelleplexes and compared their properties and transfection activity to those of the equivalent nanosphere micelleplexes and polyplexes. Article Title: N 6 -methyladenosine in 5' UTR does not promote translation initiation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins GMP-PNP Sigma Cat # G0635 TSY Pacific Biosciences 100-214-900 Cy3 mono hydrazide Cytiva PA13121 Cyanine 3.5 maleimide AAT Bioquest Cat # 149 Cy5 mono hydrazide Cytiva PA15121 2’-O-methyltransferase NEB Cat # M0336 T4 RNA ligase ThermoFisher Scientific Cat # EL0021 T4 RNA ligase 2 NEB Cat # M0242S Terminator 5ʹ-PhosphateDependent Exonuclease Lucigen Cat # TER51020 RNA 50 polyphosphatase Lucigen Cat # 136120 RNasin Ribonuclease Inhibitors Promega Cat # N251B DNase I ThermoFisher Scientific EN0521 MssI FD Therno Scientific Cat # FD1344 Protein kinase R (PKR) Abcam Cat # ab32052 Critical commercial assays T7 megascript kit Invitrogen Cat # AM1334 Vaccinia Capping System NEB Cat # M2080S HiScribe RNA synthesis kit NEB Cat # E2040S OneStep Human Coupled IVT Kit ThermoFisher Scientific Cat # 88881 Luciferase Assay System Promega Cat # Article Title: Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery Article Snippet: Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree.. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction.. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Article Title: In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging Article Snippet: Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways Article Snippet: The transfection with the plasmid pGL4.51 ( Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways. Article Snippet: Objective: The recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) to have a bi-specific oncolytic character in many tumor cells, but its action pathway in killing tumor cells has not been accurately elucidated.. Here, we studied the mechanism of apoptosis and autophagy induced by Ad-VT and the interaction between autophagy Article Title: Suppression effect of a dual cancer-specific oncolytic adenovirus on luciferase-labeled human melanoma cells in vitro and in vivo Article Snippet: BACKGROUND: To explore the suppressive effect of Apoptin-loaded oncolytic adenovirus (Ad-VT) on luciferase-labeled human melanoma cells in vitro and in vivo.. METHODS: The stable luciferase-expressing human melanoma cells A375-luc or M14-luc were obtained by transfecting the plasmid pGL4.51 and selection with G418, followed by luciferase activity, genetic stability and bioluminescence intensity assays.. In vitro, the inhibitory effects of Ad-VT on A375-luc or M14-luc were evaluated using the MTS cell proliferation, FITC-Annexin V apoptosis detection, transwell migration, Matrigel invasion and scratch assays. Recombinant:Article Title: Length-Controlled Nanofiber Micelleplexes as Efficient Nucleic Acid Delivery Vehicles. Article Snippet: Micelleplexes show great promise as effective polymeric delivery systems for nucleic acids.. Although studies have shown that spherical micelleplexes can exhibit superior cellular transfection to polyplexes, to date there has been no report on the effects of micelleplex morphology on cellular transfection.. In this work, we prepared precision, length-tunable poly(fluorenetrimethylenecarbonate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PFTMC16-b-PDMAEMA131) nanofiber micelleplexes and compared their properties and transfection activity to those of the equivalent nanosphere micelleplexes and polyplexes. Article Title: N 6 -methyladenosine in 5' UTR does not promote translation initiation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins GMP-PNP Sigma Cat # G0635 TSY Pacific Biosciences 100-214-900 Cy3 mono hydrazide Cytiva PA13121 Cyanine 3.5 maleimide AAT Bioquest Cat # 149 Cy5 mono hydrazide Cytiva PA15121 2’-O-methyltransferase NEB Cat # M0336 T4 RNA ligase ThermoFisher Scientific Cat # EL0021 T4 RNA ligase 2 NEB Cat # M0242S Terminator 5ʹ-PhosphateDependent Exonuclease Lucigen Cat # TER51020 RNA 50 polyphosphatase Lucigen Cat # 136120 RNasin Ribonuclease Inhibitors Promega Cat # N251B DNase I ThermoFisher Scientific EN0521 MssI FD Therno Scientific Cat # FD1344 Protein kinase R (PKR) Abcam Cat # ab32052 Critical commercial assays T7 megascript kit Invitrogen Cat # AM1334 Vaccinia Capping System NEB Cat # M2080S HiScribe RNA synthesis kit NEB Cat # E2040S OneStep Human Coupled IVT Kit ThermoFisher Scientific Cat # 88881 Luciferase Assay System Promega Cat # Article Title: Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery Article Snippet: Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree.. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction.. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Article Title: In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging Article Snippet: Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways Article Snippet: The transfection with the plasmid pGL4.51 ( Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways. Article Snippet: Objective: The recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) to have a bi-specific oncolytic character in many tumor cells, but its action pathway in killing tumor cells has not been accurately elucidated.. Here, we studied the mechanism of apoptosis and autophagy induced by Ad-VT and the interaction between autophagy Article Title: Suppression effect of a dual cancer-specific oncolytic adenovirus on luciferase-labeled human melanoma cells in vitro and in vivo Article Snippet: BACKGROUND: To explore the suppressive effect of Apoptin-loaded oncolytic adenovirus (Ad-VT) on luciferase-labeled human melanoma cells in vitro and in vivo.. METHODS: The stable luciferase-expressing human melanoma cells A375-luc or M14-luc were obtained by transfecting the plasmid pGL4.51 and selection with G418, followed by luciferase activity, genetic stability and bioluminescence intensity assays.. In vitro, the inhibitory effects of Ad-VT on A375-luc or M14-luc were evaluated using the MTS cell proliferation, FITC-Annexin V apoptosis detection, transwell migration, Matrigel invasion and scratch assays. Luciferase:Article Title: Length-Controlled Nanofiber Micelleplexes as Efficient Nucleic Acid Delivery Vehicles. Article Snippet: Micelleplexes show great promise as effective polymeric delivery systems for nucleic acids.. Although studies have shown that spherical micelleplexes can exhibit superior cellular transfection to polyplexes, to date there has been no report on the effects of micelleplex morphology on cellular transfection.. In this work, we prepared precision, length-tunable poly(fluorenetrimethylenecarbonate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PFTMC16-b-PDMAEMA131) nanofiber micelleplexes and compared their properties and transfection activity to those of the equivalent nanosphere micelleplexes and polyplexes. Article Title: N 6 -methyladenosine in 5' UTR does not promote translation initiation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins GMP-PNP Sigma Cat # G0635 TSY Pacific Biosciences 100-214-900 Cy3 mono hydrazide Cytiva PA13121 Cyanine 3.5 maleimide AAT Bioquest Cat # 149 Cy5 mono hydrazide Cytiva PA15121 2’-O-methyltransferase NEB Cat # M0336 T4 RNA ligase ThermoFisher Scientific Cat # EL0021 T4 RNA ligase 2 NEB Cat # M0242S Terminator 5ʹ-PhosphateDependent Exonuclease Lucigen Cat # TER51020 RNA 50 polyphosphatase Lucigen Cat # 136120 RNasin Ribonuclease Inhibitors Promega Cat # N251B DNase I ThermoFisher Scientific EN0521 MssI FD Therno Scientific Cat # FD1344 Protein kinase R (PKR) Abcam Cat # ab32052 Critical commercial assays T7 megascript kit Invitrogen Cat # AM1334 Vaccinia Capping System NEB Cat # M2080S HiScribe RNA synthesis kit NEB Cat # E2040S OneStep Human Coupled IVT Kit ThermoFisher Scientific Cat # 88881 Luciferase Assay System Promega Cat # Article Title: Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery Article Snippet: Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree.. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction.. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Article Title: In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging Article Snippet: Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways Article Snippet: The transfection with the plasmid pGL4.51 ( Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways. Article Snippet: Objective: The recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) to have a bi-specific oncolytic character in many tumor cells, but its action pathway in killing tumor cells has not been accurately elucidated.. Here, we studied the mechanism of apoptosis and autophagy induced by Ad-VT and the interaction between autophagy Article Title: Suppression effect of a dual cancer-specific oncolytic adenovirus on luciferase-labeled human melanoma cells in vitro and in vivo Article Snippet: BACKGROUND: To explore the suppressive effect of Apoptin-loaded oncolytic adenovirus (Ad-VT) on luciferase-labeled human melanoma cells in vitro and in vivo.. METHODS: The stable luciferase-expressing human melanoma cells A375-luc or M14-luc were obtained by transfecting the plasmid pGL4.51 and selection with G418, followed by luciferase activity, genetic stability and bioluminescence intensity assays.. In vitro, the inhibitory effects of Ad-VT on A375-luc or M14-luc were evaluated using the MTS cell proliferation, FITC-Annexin V apoptosis detection, transwell migration, Matrigel invasion and scratch assays. Quantitative RT-PCR:Article Title: Length-Controlled Nanofiber Micelleplexes as Efficient Nucleic Acid Delivery Vehicles. Article Snippet: Micelleplexes show great promise as effective polymeric delivery systems for nucleic acids.. Although studies have shown that spherical micelleplexes can exhibit superior cellular transfection to polyplexes, to date there has been no report on the effects of micelleplex morphology on cellular transfection.. In this work, we prepared precision, length-tunable poly(fluorenetrimethylenecarbonate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PFTMC16-b-PDMAEMA131) nanofiber micelleplexes and compared their properties and transfection activity to those of the equivalent nanosphere micelleplexes and polyplexes. Article Title: N 6 -methyladenosine in 5' UTR does not promote translation initiation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins GMP-PNP Sigma Cat # G0635 TSY Pacific Biosciences 100-214-900 Cy3 mono hydrazide Cytiva PA13121 Cyanine 3.5 maleimide AAT Bioquest Cat # 149 Cy5 mono hydrazide Cytiva PA15121 2’-O-methyltransferase NEB Cat # M0336 T4 RNA ligase ThermoFisher Scientific Cat # EL0021 T4 RNA ligase 2 NEB Cat # M0242S Terminator 5ʹ-PhosphateDependent Exonuclease Lucigen Cat # TER51020 RNA 50 polyphosphatase Lucigen Cat # 136120 RNasin Ribonuclease Inhibitors Promega Cat # N251B DNase I ThermoFisher Scientific EN0521 MssI FD Therno Scientific Cat # FD1344 Protein kinase R (PKR) Abcam Cat # ab32052 Critical commercial assays T7 megascript kit Invitrogen Cat # AM1334 Vaccinia Capping System NEB Cat # M2080S HiScribe RNA synthesis kit NEB Cat # E2040S OneStep Human Coupled IVT Kit ThermoFisher Scientific Cat # 88881 Luciferase Assay System Promega Cat # Article Title: Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery Article Snippet: Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree.. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction.. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Article Title: In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging Article Snippet: Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways Article Snippet: The transfection with the plasmid pGL4.51 ( Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways. Article Snippet: Objective: The recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) to have a bi-specific oncolytic character in many tumor cells, but its action pathway in killing tumor cells has not been accurately elucidated.. Here, we studied the mechanism of apoptosis and autophagy induced by Ad-VT and the interaction between autophagy Article Title: Suppression effect of a dual cancer-specific oncolytic adenovirus on luciferase-labeled human melanoma cells in vitro and in vivo Article Snippet: BACKGROUND: To explore the suppressive effect of Apoptin-loaded oncolytic adenovirus (Ad-VT) on luciferase-labeled human melanoma cells in vitro and in vivo.. METHODS: The stable luciferase-expressing human melanoma cells A375-luc or M14-luc were obtained by transfecting the plasmid pGL4.51 and selection with G418, followed by luciferase activity, genetic stability and bioluminescence intensity assays.. In vitro, the inhibitory effects of Ad-VT on A375-luc or M14-luc were evaluated using the MTS cell proliferation, FITC-Annexin V apoptosis detection, transwell migration, Matrigel invasion and scratch assays. Software:Article Title: Length-Controlled Nanofiber Micelleplexes as Efficient Nucleic Acid Delivery Vehicles. Article Snippet: Micelleplexes show great promise as effective polymeric delivery systems for nucleic acids.. Although studies have shown that spherical micelleplexes can exhibit superior cellular transfection to polyplexes, to date there has been no report on the effects of micelleplex morphology on cellular transfection.. In this work, we prepared precision, length-tunable poly(fluorenetrimethylenecarbonate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PFTMC16-b-PDMAEMA131) nanofiber micelleplexes and compared their properties and transfection activity to those of the equivalent nanosphere micelleplexes and polyplexes. Article Title: N 6 -methyladenosine in 5' UTR does not promote translation initiation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins GMP-PNP Sigma Cat # G0635 TSY Pacific Biosciences 100-214-900 Cy3 mono hydrazide Cytiva PA13121 Cyanine 3.5 maleimide AAT Bioquest Cat # 149 Cy5 mono hydrazide Cytiva PA15121 2’-O-methyltransferase NEB Cat # M0336 T4 RNA ligase ThermoFisher Scientific Cat # EL0021 T4 RNA ligase 2 NEB Cat # M0242S Terminator 5ʹ-PhosphateDependent Exonuclease Lucigen Cat # TER51020 RNA 50 polyphosphatase Lucigen Cat # 136120 RNasin Ribonuclease Inhibitors Promega Cat # N251B DNase I ThermoFisher Scientific EN0521 MssI FD Therno Scientific Cat # FD1344 Protein kinase R (PKR) Abcam Cat # ab32052 Critical commercial assays T7 megascript kit Invitrogen Cat # AM1334 Vaccinia Capping System NEB Cat # M2080S HiScribe RNA synthesis kit NEB Cat # E2040S OneStep Human Coupled IVT Kit ThermoFisher Scientific Cat # 88881 Luciferase Assay System Promega Cat # Article Title: Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery Article Snippet: Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree.. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction.. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Article Title: In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging Article Snippet: Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways Article Snippet: The transfection with the plasmid pGL4.51 ( Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways. Article Snippet: Objective: The recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) to have a bi-specific oncolytic character in many tumor cells, but its action pathway in killing tumor cells has not been accurately elucidated.. Here, we studied the mechanism of apoptosis and autophagy induced by Ad-VT and the interaction between autophagy Article Title: Suppression effect of a dual cancer-specific oncolytic adenovirus on luciferase-labeled human melanoma cells in vitro and in vivo Article Snippet: BACKGROUND: To explore the suppressive effect of Apoptin-loaded oncolytic adenovirus (Ad-VT) on luciferase-labeled human melanoma cells in vitro and in vivo.. METHODS: The stable luciferase-expressing human melanoma cells A375-luc or M14-luc were obtained by transfecting the plasmid pGL4.51 and selection with G418, followed by luciferase activity, genetic stability and bioluminescence intensity assays.. In vitro, the inhibitory effects of Ad-VT on A375-luc or M14-luc were evaluated using the MTS cell proliferation, FITC-Annexin V apoptosis detection, transwell migration, Matrigel invasion and scratch assays. In Vivo:Article Title: Length-Controlled Nanofiber Micelleplexes as Efficient Nucleic Acid Delivery Vehicles. Article Snippet: Micelleplexes show great promise as effective polymeric delivery systems for nucleic acids.. Although studies have shown that spherical micelleplexes can exhibit superior cellular transfection to polyplexes, to date there has been no report on the effects of micelleplex morphology on cellular transfection.. In this work, we prepared precision, length-tunable poly(fluorenetrimethylenecarbonate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PFTMC16-b-PDMAEMA131) nanofiber micelleplexes and compared their properties and transfection activity to those of the equivalent nanosphere micelleplexes and polyplexes. Article Title: N 6 -methyladenosine in 5' UTR does not promote translation initiation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins GMP-PNP Sigma Cat # G0635 TSY Pacific Biosciences 100-214-900 Cy3 mono hydrazide Cytiva PA13121 Cyanine 3.5 maleimide AAT Bioquest Cat # 149 Cy5 mono hydrazide Cytiva PA15121 2’-O-methyltransferase NEB Cat # M0336 T4 RNA ligase ThermoFisher Scientific Cat # EL0021 T4 RNA ligase 2 NEB Cat # M0242S Terminator 5ʹ-PhosphateDependent Exonuclease Lucigen Cat # TER51020 RNA 50 polyphosphatase Lucigen Cat # 136120 RNasin Ribonuclease Inhibitors Promega Cat # N251B DNase I ThermoFisher Scientific EN0521 MssI FD Therno Scientific Cat # FD1344 Protein kinase R (PKR) Abcam Cat # ab32052 Critical commercial assays T7 megascript kit Invitrogen Cat # AM1334 Vaccinia Capping System NEB Cat # M2080S HiScribe RNA synthesis kit NEB Cat # E2040S OneStep Human Coupled IVT Kit ThermoFisher Scientific Cat # 88881 Luciferase Assay System Promega Cat # Article Title: Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery Article Snippet: Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree.. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction.. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Article Title: In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging Article Snippet: Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways Article Snippet: The transfection with the plasmid pGL4.51 ( Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways. Article Snippet: Objective: The recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) to have a bi-specific oncolytic character in many tumor cells, but its action pathway in killing tumor cells has not been accurately elucidated.. Here, we studied the mechanism of apoptosis and autophagy induced by Ad-VT and the interaction between autophagy Article Title: Suppression effect of a dual cancer-specific oncolytic adenovirus on luciferase-labeled human melanoma cells in vitro and in vivo Article Snippet: BACKGROUND: To explore the suppressive effect of Apoptin-loaded oncolytic adenovirus (Ad-VT) on luciferase-labeled human melanoma cells in vitro and in vivo.. METHODS: The stable luciferase-expressing human melanoma cells A375-luc or M14-luc were obtained by transfecting the plasmid pGL4.51 and selection with G418, followed by luciferase activity, genetic stability and bioluminescence intensity assays.. In vitro, the inhibitory effects of Ad-VT on A375-luc or M14-luc were evaluated using the MTS cell proliferation, FITC-Annexin V apoptosis detection, transwell migration, Matrigel invasion and scratch assays. Purification:Article Title: Length-Controlled Nanofiber Micelleplexes as Efficient Nucleic Acid Delivery Vehicles. Article Snippet: Micelleplexes show great promise as effective polymeric delivery systems for nucleic acids.. Although studies have shown that spherical micelleplexes can exhibit superior cellular transfection to polyplexes, to date there has been no report on the effects of micelleplex morphology on cellular transfection.. In this work, we prepared precision, length-tunable poly(fluorenetrimethylenecarbonate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PFTMC16-b-PDMAEMA131) nanofiber micelleplexes and compared their properties and transfection activity to those of the equivalent nanosphere micelleplexes and polyplexes. Article Title: N 6 -methyladenosine in 5' UTR does not promote translation initiation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins GMP-PNP Sigma Cat # G0635 TSY Pacific Biosciences 100-214-900 Cy3 mono hydrazide Cytiva PA13121 Cyanine 3.5 maleimide AAT Bioquest Cat # 149 Cy5 mono hydrazide Cytiva PA15121 2’-O-methyltransferase NEB Cat # M0336 T4 RNA ligase ThermoFisher Scientific Cat # EL0021 T4 RNA ligase 2 NEB Cat # M0242S Terminator 5ʹ-PhosphateDependent Exonuclease Lucigen Cat # TER51020 RNA 50 polyphosphatase Lucigen Cat # 136120 RNasin Ribonuclease Inhibitors Promega Cat # N251B DNase I ThermoFisher Scientific EN0521 MssI FD Therno Scientific Cat # FD1344 Protein kinase R (PKR) Abcam Cat # ab32052 Critical commercial assays T7 megascript kit Invitrogen Cat # AM1334 Vaccinia Capping System NEB Cat # M2080S HiScribe RNA synthesis kit NEB Cat # E2040S OneStep Human Coupled IVT Kit ThermoFisher Scientific Cat # 88881 Luciferase Assay System Promega Cat # Article Title: Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery Article Snippet: Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree.. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction.. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Article Title: In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging Article Snippet: Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways Article Snippet: The transfection with the plasmid pGL4.51 ( Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways. Article Snippet: Objective: The recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) to have a bi-specific oncolytic character in many tumor cells, but its action pathway in killing tumor cells has not been accurately elucidated.. Here, we studied the mechanism of apoptosis and autophagy induced by Ad-VT and the interaction between autophagy Article Title: Suppression effect of a dual cancer-specific oncolytic adenovirus on luciferase-labeled human melanoma cells in vitro and in vivo Article Snippet: BACKGROUND: To explore the suppressive effect of Apoptin-loaded oncolytic adenovirus (Ad-VT) on luciferase-labeled human melanoma cells in vitro and in vivo.. METHODS: The stable luciferase-expressing human melanoma cells A375-luc or M14-luc were obtained by transfecting the plasmid pGL4.51 and selection with G418, followed by luciferase activity, genetic stability and bioluminescence intensity assays.. In vitro, the inhibitory effects of Ad-VT on A375-luc or M14-luc were evaluated using the MTS cell proliferation, FITC-Annexin V apoptosis detection, transwell migration, Matrigel invasion and scratch assays. Transformation Assay:Article Title: Length-Controlled Nanofiber Micelleplexes as Efficient Nucleic Acid Delivery Vehicles. Article Snippet: Micelleplexes show great promise as effective polymeric delivery systems for nucleic acids.. Although studies have shown that spherical micelleplexes can exhibit superior cellular transfection to polyplexes, to date there has been no report on the effects of micelleplex morphology on cellular transfection.. In this work, we prepared precision, length-tunable poly(fluorenetrimethylenecarbonate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PFTMC16-b-PDMAEMA131) nanofiber micelleplexes and compared their properties and transfection activity to those of the equivalent nanosphere micelleplexes and polyplexes. Article Title: N 6 -methyladenosine in 5' UTR does not promote translation initiation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins GMP-PNP Sigma Cat # G0635 TSY Pacific Biosciences 100-214-900 Cy3 mono hydrazide Cytiva PA13121 Cyanine 3.5 maleimide AAT Bioquest Cat # 149 Cy5 mono hydrazide Cytiva PA15121 2’-O-methyltransferase NEB Cat # M0336 T4 RNA ligase ThermoFisher Scientific Cat # EL0021 T4 RNA ligase 2 NEB Cat # M0242S Terminator 5ʹ-PhosphateDependent Exonuclease Lucigen Cat # TER51020 RNA 50 polyphosphatase Lucigen Cat # 136120 RNasin Ribonuclease Inhibitors Promega Cat # N251B DNase I ThermoFisher Scientific EN0521 MssI FD Therno Scientific Cat # FD1344 Protein kinase R (PKR) Abcam Cat # ab32052 Critical commercial assays T7 megascript kit Invitrogen Cat # AM1334 Vaccinia Capping System NEB Cat # M2080S HiScribe RNA synthesis kit NEB Cat # E2040S OneStep Human Coupled IVT Kit ThermoFisher Scientific Cat # 88881 Luciferase Assay System Promega Cat # Article Title: Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery Article Snippet: Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree.. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction.. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Article Title: In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging Article Snippet: Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways Article Snippet: The transfection with the plasmid pGL4.51 ( Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways. Article Snippet: Objective: The recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) to have a bi-specific oncolytic character in many tumor cells, but its action pathway in killing tumor cells has not been accurately elucidated.. Here, we studied the mechanism of apoptosis and autophagy induced by Ad-VT and the interaction between autophagy Article Title: Suppression effect of a dual cancer-specific oncolytic adenovirus on luciferase-labeled human melanoma cells in vitro and in vivo Article Snippet: BACKGROUND: To explore the suppressive effect of Apoptin-loaded oncolytic adenovirus (Ad-VT) on luciferase-labeled human melanoma cells in vitro and in vivo.. METHODS: The stable luciferase-expressing human melanoma cells A375-luc or M14-luc were obtained by transfecting the plasmid pGL4.51 and selection with G418, followed by luciferase activity, genetic stability and bioluminescence intensity assays.. In vitro, the inhibitory effects of Ad-VT on A375-luc or M14-luc were evaluated using the MTS cell proliferation, FITC-Annexin V apoptosis detection, transwell migration, Matrigel invasion and scratch assays. Control:Article Title: Length-Controlled Nanofiber Micelleplexes as Efficient Nucleic Acid Delivery Vehicles. Article Snippet: Micelleplexes show great promise as effective polymeric delivery systems for nucleic acids.. Although studies have shown that spherical micelleplexes can exhibit superior cellular transfection to polyplexes, to date there has been no report on the effects of micelleplex morphology on cellular transfection.. In this work, we prepared precision, length-tunable poly(fluorenetrimethylenecarbonate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PFTMC16-b-PDMAEMA131) nanofiber micelleplexes and compared their properties and transfection activity to those of the equivalent nanosphere micelleplexes and polyplexes. Article Title: N 6 -methyladenosine in 5' UTR does not promote translation initiation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins GMP-PNP Sigma Cat # G0635 TSY Pacific Biosciences 100-214-900 Cy3 mono hydrazide Cytiva PA13121 Cyanine 3.5 maleimide AAT Bioquest Cat # 149 Cy5 mono hydrazide Cytiva PA15121 2’-O-methyltransferase NEB Cat # M0336 T4 RNA ligase ThermoFisher Scientific Cat # EL0021 T4 RNA ligase 2 NEB Cat # M0242S Terminator 5ʹ-PhosphateDependent Exonuclease Lucigen Cat # TER51020 RNA 50 polyphosphatase Lucigen Cat # 136120 RNasin Ribonuclease Inhibitors Promega Cat # N251B DNase I ThermoFisher Scientific EN0521 MssI FD Therno Scientific Cat # FD1344 Protein kinase R (PKR) Abcam Cat # ab32052 Critical commercial assays T7 megascript kit Invitrogen Cat # AM1334 Vaccinia Capping System NEB Cat # M2080S HiScribe RNA synthesis kit NEB Cat # E2040S OneStep Human Coupled IVT Kit ThermoFisher Scientific Cat # 88881 Luciferase Assay System Promega Cat # Article Title: Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery Article Snippet: Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree.. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction.. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Article Title: In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging Article Snippet: Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways Article Snippet: The transfection with the plasmid pGL4.51 ( Article Title: Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways. Article Snippet: Objective: The recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) to have a bi-specific oncolytic character in many tumor cells, but its action pathway in killing tumor cells has not been accurately elucidated.. Here, we studied the mechanism of apoptosis and autophagy induced by Ad-VT and the interaction between autophagy Article Title: Suppression effect of a dual cancer-specific oncolytic adenovirus on luciferase-labeled human melanoma cells in vitro and in vivo Article Snippet: BACKGROUND: To explore the suppressive effect of Apoptin-loaded oncolytic adenovirus (Ad-VT) on luciferase-labeled human melanoma cells in vitro and in vivo.. METHODS: The stable luciferase-expressing human melanoma cells A375-luc or M14-luc were obtained by transfecting the plasmid pGL4.51 and selection with G418, followed by luciferase activity, genetic stability and bioluminescence intensity assays.. In vitro, the inhibitory effects of Ad-VT on A375-luc or M14-luc were evaluated using the MTS cell proliferation, FITC-Annexin V apoptosis detection, transwell migration, Matrigel invasion and scratch assays. |
![( A ) HT29 <t>Luc2</t> control (Control; Luc shRNA); HDAC5 knockdown (HDAC5kd; HDAC5 shRNA); PARP1 knockdown (PARP1kd; PARP1 shRNA); PARP1 knockdown cells (PARP1kd; PARP1 shRNA) expressing PARP1 K498R (PARP1kd/PARP1 K498R ), PARP1 K521R (PARP1kd/PARP1 K521R ), or PARP1 K498R,521R (PARP1kd/PARP1 K498R,521R ); and HDAC5 knockdown cells expressing HDAC5 S259/498A (HDAC5kd/HDAC5 S259/498A ) were orthotopically injected into the cecum wall of nude mice. After 1 week, mice were administered 5FU (10 mg/kg) every alternate day. Olaparib was also coadministered at a dose of 10 mg/kg daily as indicated. Bioluminescence imaging was performed. ( B ) Bioluminescence quantification [(A) above]. The data are representative of three independent experiments using five individual mice per group. Error bars are means ± SD from five individual mice ( n = 5 mice per group). Statistical analyses were done using two-way ANOVA (Tukey’s post hoc test). *** P < 0.001. ( C and D ) At the end of 5 weeks, the liver and lung from indicated mice [(A) above] were analyzed by ex vivo imaging (C) and hematoxylin and eosin staining (D). ( E ) At the end of 5 weeks, the blood from mice in (A) above was used to isolate genomic DNA for examining circulating tumor cells. The data are representative of three independent experiments using five individual mice per group. Error bars are means ± SD from five individual mice ( n = 5 mice per group) * P < 0.05; ** P < 0.01; *** P < 0.001. ( F ) At the end of 5 weeks, lysates of primary orthotopic tumors from (A) above were analyzed by immunoblotting for the indicated proteins. ( G and H ) Representative image of immunostaining of the indicated proteins in different grades of colon adenocarcinoma and matched normal adjacent tissue (NAT) (G). Quantitation of HDAC5, AcK498-PARP1, and AcK521-PARP1 levels (H). Statistical analyses were done using one-way ANOVA (Dunn’s multiple comparison test). Error bars are means ± SD. ** P < 0.01; *** P < 0.001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8539/pmc11488539/pmc11488539__sciadv.ado7720-f7.jpg)